In-vitro interaction of daminozide with some amino acids

In this study, the interaction of plant growth regulatory substance daminozide with alanine, arginine, glycine, glutamic acid, histidine, isoleucine, methionine, phenylalanine, threonine, tryptophan, tyrosine and valine has been investigated by thin-layer chromatography. In the experiment 100, 200 and 400 ppm doses of daminozide has been used. At the end of experiments with or without for 24 hours, in the Retention factor (Rf ) value, according to standard amino acids values, some increasing or decreasing values have been observed. Moreover some alterations in maximum absorption peaks on the spectrums taken from thin-layer cromotgraphy samples have been observed. It has been observed that there were some changes in amino acids with different daminozide doses. The obtained results show that daminozide either will be able to bound to amino acids or form new copounds under in-vitro conditions.